Temperature Controlled Microscope Stages


CMS196 - CRYO-CORRELATIVE MICROSCOPY STAGE

Developed in collaboration with Prof. Bram Koster's team at Leiden University Medical Centre (LUMC) in the Netherlands, the CMS196 is the result of 4 years of R&D.  

“The correlative stage was developed out of the necessity for a tool that uses fluorescence microscopy to help localize structures of interest in samples for high resolution cryo electron  microscopy imaging. Over the years the system has evolved into an effective and efficient instrument that enables the imaging of cryo electron microscope images after light microscopy characterization routinely.”  Prof. A.J. Koster - LUMC

Features / Highlights of the CMS196M

  • Self-contained cryo sample observation system with automatic top-up of the sample chamber with liquid nitrogen, keeping the sample vitrified at constant -196°C and avoiding contamination
  • Short startup time of only 10 minutes, no mechanical connection between sample mount and dewar, very good stability, low drift in nm range, good long-term stability
  • Integrated motorised XY stage with optical encoders for high precision movement and position readout of better than 1um
  • Fully Automated XY Mapping
  • Mounting option for most upright microscopes from all major manufacturers
  • Self-aligning magnetic sample cassette system for up to three samples
  • Support for EM-grids, Auto-Grids (FEI), Membrane carriers (Leica HPM100), Bessy grids, planchettes, CryoCapsule from CryoCapCell and custom sample holders, no need to handle bare grids
  • Sample cassette holder for safe and contamination-free sample loading, storage and transfer
  • Connects to plunge-freezing and High-Pressure Freezing sample preparation protocols
  • Integrated sensors for sample temperature monitoring
  • Integrated condenser optics for transmitted light brightfield and phase contrast
  • Fluorescence observation with NA of up to 0.9
  • LN2 refill cycle of 35 minutes with integrated dewar, full day of independent operation with linked 3l autofill dewar
  • LCD display with button control, joystick operation (option) and control via USB interface
  • Automated heating cycle to drive out moisture
  • More than 25 systems proven in the field worldwide, market leader in Cryo-CLEM